Click to jump to softwares you interested

Whole genome annotation is the process of identifying features of interest in a set of genomic DNA sequences, and labelling them with useful information. Prokka is a software tool to annotate bacterial, archaeal and viral genomes quickly and produce standards-compliant output files.

> Software Tutorial

Prokka

To annotate prokaryotic genome
Use Test Data and Preset Parameters

Contigs to annotate

Upload a query fasta file. (Required)

FASTA format

Locus tag prefix (--locustag)

Locus tag counter increment (--increment)

GFF version (--gffver)

Force GenBank/ENA/DDJB compliance (--compliant)

Equivalent to --addgenes --mincontiglen 200 --centre Prokka (or other centre specified below)

Add 'gene' features for each 'CDS' feature (--addgenes)

Minimum contig size (--mincontiglen)

NCBI needs 200

Sequencing centre ID (--centre)

Genus name (--genus)

May be used to aid annotation, see --usegenus below

Species name (--species)

Strain name (--strain)

Plasmid name or identifier (--plasmid)

Kingdom (--kingdom)

Use genus-specific BLAST database (--usegenus)

Optional FASTA file of trusted proteins to first annotate from (--proteins)

Upload a optional reference fasta file.

Citation(s)

Seemann T. Prokka: rapid prokaryotic genome annotation Bioinformatics 2014 Jul 15;30(14):2068-9. PMID:24642063