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Brucellosis Incidence
Brucella Prevalence
Software Tutorial
Prokka
Prokaryotic genome annotation
snippy
Snippy finds SNPs between a haploid reference genome and your NGS sequence reads
ABRicate
Mass screening of contigs for antimicrobial and virulence genes
FASTTREE
build maximum-likelihood phylogenetic trees
Shovill
Assemble bacterial isolate genomes from Illumina paired-end reads
Shovill
Use Test Data and Preset Parameters
Forward reads (R1)
Upload a fastq file. (Required)
upload
The file of forward reads in FASTQ format
Reverse reads (R2)
Upload a fastq file. (Required)
upload
The file of reverse reads in FASTQ format
Trim reads
Yes
No
Use Trimmomatic to remove common adaptors first (default: OFF) (--trim)
Contig name format
Format of contig FASTA IDs in 'printf' style (default: 'contig%05d') (--namefmt)
contig%05d
Depth
Sub-sample --R1/--R2 to this depth. Disable with --depth 0 (default: 150) (--depth)
150
Minimum contig length
Minimum length of contig to be output. 0 is AUTO (default: 0) (--minlen)
0
Minimum contig coverage
Minimum coverage to call part of a contig. 0 is AUTO (default: 2) (--mincov)
2
Citation(s)
Seemann, T. (2017).
Shovill: Faster SPAdes assembly of Illumina reads.
https://github.com/tseemann/shovill